Anticorps Recombinant de lapin anti-CCL12/MCP-5

CCL12/MCP-5 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250386A9

N° de cat : 86565-1-RR

Synonymes

C-C motif chemokine 12, Ccl12, MCP-1-related chemokine, Mcp5, MCP-5

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Galerie de données de validation

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  WB analysis using 86565-1-RR

  Non-transfected and CCL12-transfected HEK-293T cell lysates were subjected to SDS PAGE followed by western blot with 86565-1-RR (Ccl12 antibody) at a dilution of 1:5000, incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: Transfected with Mouse Ccl12 Transfected HEK-293T cells,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86565-1-RR cible CCL12/MCP-5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCL12/MCP-5 Protéine recombinante Eg3315
• Nom complet: chemokine (C-C motif) ligand 12
• Masse moléculaire calculée: 12KD
• Numéro d’acquisition GenBank: NM_011331.2
• Symbole du gène: Ccl12
• Identification du gène (NCBI): 20293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCL12 (monocyte chemotactic protein-5, MCP-5) is a murine CC-chemokine encoded within the chemokine cluster on chromosome 11. It is the closest murine homologue of human MCP-1 (CCL2), sharing 66 % amino-acid identity, and signals exclusively through the G-protein-coupled receptor CCR2. CCL12 is produced by stromal cells, astrocytes, macrophages and, under inflammatory conditions, by lung and bone-marrow stromal cells. Its receptor, CCR2, is expressed on monocytes, memory T cells, NK cells, dendritic cells and basophils. Consequently, CCL12 potently chemoattracts monocytes, lymphocytes, eosinophils and bone-marrow-derived fibrocytes, but not neutrophils.

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