Anticorps Recombinant de lapin anti-CCL22/MDC

CCL22/MDC Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

250115D3

N° de cat : 98501-2-PBS

Synonymes

Abcd1, Activated B and dendritic cell-derived, CC chemokine ABCD-1, C-C motif chemokine 22, Ccl22

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using 98501-2-RR (same clone as 98501-2-PBS)

  1x10^6 HEK-293T cells or CCL22/MDC transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL22/MDC Rabbit RecAb (98501-2-RR, Clone: 250115D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98501-2-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected HEK-293T using 98501-2-RR (same clone as 98501-2-PBS)

  1x10^6 HEK-293T cells or CCL22/MDC transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL22/MDC Rabbit RecAb (98501-2-RR, Clone: 250115D3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98501-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98501-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98501-2-RR against Mouse CCL22/MDC were performed. The affinity constant is 0.914 nM.

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Informations sur le produit

98501-2-PBS cible CCL22/MDC dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCL22/MDC Protéine recombinante Eg2241
• Nom complet: chemokine (C-C motif) ligand 22
• Masse moléculaire calculée: 10kd
• Numéro d’acquisition GenBank: NM_009137
• Symbole du gène: CCL22/MDC
• Identification du gène (NCBI): 20299
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

The C-C motif chemokine ligand 22 (CCL22), also known as MDC, belongs to the group of chemokines, that are both constitutively expressed under homeostatic conditions and inducible upon inflammation. CCL22 is a secreted protein that exerts chemotactic activity for monocytes, dendritic cells, natural killer cells, and for chronically activated T lymphocytes. CCL22 is induced by LPS, IL-4, and IL-13 and in T cells by TCR stimulation. Accumulating studies indicate that CCL22 recruits regulatory T (T-reg) cells into tumor tissues and is expressed in many human tumors.

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