Anticorps Polyclonal de lapin anti-CCL28

CCL28 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18214-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human spleen using 18214-1-AP (same clone as 18214-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 18214-1-AP (same clone as 18214-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 18214-1-AP (same clone as 18214-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 18214-1-AP (same clone as 18214-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 18214-1-AP (same clone as 18214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18214-1-AP (CCL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 18214-1-AP (same clone as 18214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18214-1-AP (CCL28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18214-1-PBS cible CCL28 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCL28 Protéine recombinante Ag12920
• Nom complet: chemokine (C-C motif) ligand 28
• Masse moléculaire calculée: 127 aa, 14 kDa
• Poids moléculaire observé: 8 kDa-9 kDa
• Numéro d’acquisition GenBank: BC069532
• Symbole du gène: CCL28
• Identification du gène (NCBI): 56477
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCL28, also named as SCYA28, CCK1 and MEC, belongs to the intercrine beta (chemokine CC) family. It has chemotactic activity for resting CD4, CD8 T-cells and eosinophils. CCL28 binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. CCL28 is expressed in a variety of human and mouse tissues, and it appears to be predominantly produced by epithelial cells. It is produced by epithelial cells of these tissues suggesting that this chemokine can play an important role by linking homing mechanisms between the gut, nasal mucosa and mammary gland (MG). In WB test, CCL28 is 14kd and 8-9kd(a putatively to a degradation fragment).

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