Anticorps Polyclonal de lapin anti-CCL3/MIP-1 alpha

CCL3/MIP-1 alpha Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 32190-1-AP

Synonymes

C-C motif chemokine 3, Ccl3, Heparin-binding chemotaxis protein, L2G25B, Macrophage inflammatory protein 1-alpha

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Galerie de données de validation

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  WB analysis using 32190-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32190-1-AP (CCL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 32190-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using CCL3 antibody (32190-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7 traitées au LPS et à la bréfeldine A,
• Résultats positifs en IF/ICC: PMA, LPS and Brefeldin A treated THP-1 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32190-1-AP cible CCL3/MIP-1 alpha dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCL3/MIP-1 alpha Protéine recombinante Eg2170
• Nom complet: chemokine (C-C motif) ligand 3
• Masse moléculaire calculée: 10kd
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: NM_011337
• Symbole du gène: Ccl3
• Identification du gène (NCBI): 20302
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1α, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.

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