Anticorps Recombinant de lapin anti-CCL7/MCP-3

CCL7/MCP-3 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

250662G6

N° de cat : 98555-1-RR

Synonymes

C-C motif chemokine 7, Ccl7, Fic, Intercrine/chemokine MARC, Mcp3

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Galerie de données de validation

• 

  FC experiment of Transfected HEK-293T using 98555-1-RR

  1x10^6 HEK-293T cells or CCL7/MCP-3 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL7/MCP-3 Rabbit RecAb (98555-1-RR, Clone: 250662G6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

• 

  FC experiment of Transfected HEK-293T using 98555-1-RR

  1x10^6 HEK-293T cells or CCL7/MCP-3 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL7/MCP-3 Rabbit RecAb (98555-1-RR, Clone:250662G6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

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Applications testées

• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98555-1-RR cible CCL7/MCP-3 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCL7/MCP-3 Protéine recombinante Eg4626
• Nom complet: chemokine (C-C motif) ligand 7
• Masse moléculaire calculée: 11kDa
• Numéro d’acquisition GenBank: NM_013654.3
• Symbole du gène: Ccl7
• Identification du gène (NCBI): 20306
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

The chemokine CCL7 (MCP3) is a chemotactic factor and potent attractant of monocytes firstly characterized from the culture supernatants of MG-63 osteosarcoma cells. CCL7 is known to promote the recruitment of many innate immune cell types including monocytes and neutrophils to sites of bacterial and viral infection and eosinophils and basophils to sites of allergic inflammation. CCL7 is expressed at low levels in endothelial cells, fibroblasts and mononuclear cells and upregulated by various stimuli including viruses, type I or type II interferons (IFNs). CCL7 (MCP-3) mediates effects on a host of innate and adaptive immune cell types through binding to numerous receptors including CCR1, CCR2, CCR3, CCR5, and CCR10.

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