Anticorps Polyclonal de lapin anti-Cyclin D2

• Phare
• Validé par KD/KO

Cyclin D2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

46

N° de cat : 10934-1-AP

Synonymes

CCND2, cyclin D, cyclin D2, G1/S-specific cyclin-D2

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Galerie de données de validation

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  WB analysis using 10934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10934-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10934-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10934-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10934-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10934-1-AP (Cyclin D2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10934-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10934-1-AP (Cyclin D2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HEK-293, cellules MCF-7, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 38 publications below

Informations sur le produit

10934-1-AP cible Cyclin D2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: bovin, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclin D2 Protéine recombinante Ag1377
• Nom complet: cyclin D2
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC010958
• Symbole du gène: CCND2
• Identification du gène (NCBI): 894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.

Publications

Species	Application	Title
mouse		Nature; SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma.; Authors - Soufiane Boumahdi
human	WB	Cancer Cell; The Oncogenic Transcription Factor RUNX1/ETO Corrupts Cell Cycle Regulation to Drive Leukemic Transformation.; Authors - Natalia Martinez-Soria ; KD Validated
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin
mouse	WB	Dev Cell; Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.; Authors - Yan Gong
mouse		Kidney Int; Transcription factor MafB may play an important role in secondary hyperparathyroidism.; Authors - Naoki Morito
human	WB	Int J Cancer; Human tumor microRNA signatures derived from large-scale oligonucleotide microarray datasets.; Authors - Wang Wenzhang W

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