Anticorps Polyclonal de lapin anti-Cyclin D3

Cyclin D3 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10845-1-AP

Synonymes

CCND3, Cyclin D3

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Galerie de données de validation

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  WB analysis of MDA-MB-453s using 10845-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10845-1-AP (CCND3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

10845-1-AP cible Cyclin D3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclin D3 Protéine recombinante Ag1292
• Nom complet: cyclin D3
• Masse moléculaire calculée: 33 kDa
• Numéro d’acquisition GenBank: BC011616
• Symbole du gène: CCND3
• Identification du gène (NCBI): 896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Publications

Species	Application	Title
human	WB	Oncogene; USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation; Authors - Cunying Ma
human	WB	FASEB J; C1orf61 acts as a tumor activator in human hepatocellular carcinoma and is associated with tumorigenesis and metastasis.; Authors - Hu Hai-Ming HM
human	WB	J Biol Chem; Sj7170, a unique dual-functional peptide with a specific α-chymotrypsin inhibitory activity and a potent tumor activating effect from scorpion venom.; Authors - Yu Song
human	WB	Phytomedicine; Antileukemic effect of caffeic acid 3,4-dihydroxyphenetyl ester. Evidences for its mechanisms of action.; Authors - Mingmin Tang
human	WB,IHC	BMC Cancer; PMID: 16978399; Authors - Zhang Ping P
		Int J Oncol; Tetrandrine induces G1/S cell cycle arrest through the ROS/Akt pathway in EOMA cells and inhibits angiogenesis in vivo.; Authors - Wenkai Xiao