Anticorps Polyclonal de lapin anti-Cyclin E2

Cyclin E2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(61)

Avis (1)

N° de cat : 11935-1-AP

Synonymes

CCNE2, CYCE2, Cyclin E, G1/S specific cyclin E2, G1/S-specific cyclin-E2

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Galerie de données de validation

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  IP experiment of Jurkat using 11935-1-AP

  IP result of anti-Cyclin E2 (IP:11935-1-AP, 4ug; Detection:11935-1-AP 1:300) with Jurkat cells lysate 4000ug.

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  IHC staining of human breast cancer using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11935-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11935-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin E2 antibody (11935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 11935-1-AP

  Immunofluorescent analysis of HeLa cells using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HeLa using 11935-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E2 Polyclonal antibody (11935-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 56 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

11935-1-AP cible Cyclin E2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclin E2 Protéine recombinante Ag2532
• Nom complet: cyclin E2
• Masse moléculaire calculée: 374 aa, 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC020729
• Symbole du gène: CCNE2
• Identification du gène (NCBI): 9134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regeneration; Authors - Dongcheng Cai
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin
mouse	IHC	Theranostics; Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction; Authors - Meng Gao
mouse,human	WB	Cell Commun Signal; Decursin induces FLT3-ITD acute myeloid leukemia cell apoptosis by increasing the expression of the ubiquitin-conjugase UBE2L6; Authors - Tianxin Zhang
human	WB	Elife; PMID: 30132758; Authors - Tonya Kueck
human	IHC	Mol Ther Nucleic Acids; miRNA in food simultaneously controls animal viral disease and human tumorigenesis.; Authors - Yalei Cui

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Juri (Verified Customer) (12-22-2023)

Worked ok with immunocytochemistry

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 in 200
• Cell Tissue Type: Cell

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