Anticorps Recombinant de lapin anti-CCR2a-specific

CCR2a-specific Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242217G4

N° de cat : 84911-3-RR

Synonymes

CCR2, 242217G4, C C chemokine receptor type 2, C C CKR 2, C-C chemokine receptor type 2

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Galerie de données de validation

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  WB analysis using 84911-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84911-3-RR (CCR2a-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 84911-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84911-3-RR (CCR2a-specific antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 84911-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84911-3-RR (CCR2a-specific antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 84911-3-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CCR2a-specific antibody (84911-3-RR, Clone: 242217G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules U-937, cellules A431, cellules Jurkat, cellules THP-1
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84911-3-RR cible CCR2a-specific dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: chemokine (C-C motif) receptor 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_000647
• Symbole du gène: CCR2
• Identification du gène (NCBI): 729230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates.

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