Anticorps Monoclonal anti-CCS

• Phare
• Validé par KD/KO

CCS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F5E5

N° de cat : 68341-1-PBS

Synonymes

CCS

---

Galerie de données de validation

• 

  WB analysis using 68341-1-Ig (same clone as 68341-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68341-1-Ig (CCS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

  WB result of CCS antibody (68341-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCS transfected HepG2 cells. This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68341-1-Ig (same clone as 68341-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68341-1-Ig (CCS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCS antibody (68341-1-Ig, Clone: 3F5E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68341-1-PBS cible CCS dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCS Protéine recombinante Ag18860
• Nom complet: copper chaperone for superoxide dismutase
• Masse moléculaire calculée: 274 aa, 29 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC105016
• Symbole du gène: CCS
• Identification du gène (NCBI): 9973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.

{{ptg:RelatedPrimaryAntibodies}}