Anticorps Polyclonal de lapin anti-CCT2

• Phare
• Validé par KD/KO

CCT2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24896-1-PBS

Synonymes

PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta

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Galerie de données de validation

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  WB analysis of mouse testis using 24896-1-AP (same clone as 24896-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 24896-1-AP (same clone as 24896-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 24896-1-AP (same clone as 24896-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 24896-1-AP (same clone as 24896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24896-1-AP (CCT2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 24896-1-AP (same clone as 24896-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CCT2 antibody (24896-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24896-1-PBS cible CCT2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCT2 Protéine recombinante Ag19264
• Nom complet: chaperonin containing TCP1, subunit 2 (beta)
• Masse moléculaire calculée: 535 aa, 57 kDa
• Poids moléculaire observé: 50-58 kDa
• Numéro d’acquisition GenBank: BC113516
• Symbole du gène: CCT2
• Identification du gène (NCBI): 10576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)

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