Anticorps Monoclonal anti-CCT2

CCT2 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2H6C7

N° de cat : 68214-1-PBS

Synonymes

99D8.1, CCT beta, CCTB, CCT-beta, Chaperonin containing T-complex polypeptide 1 subunit 2

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Galerie de données de validation

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  WB analysis using 68214-1-Ig (same clone as 68214-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68214-1-Ig (CCT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68214-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 68214-1-Ig (same clone as 68214-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CCT2 antibody (68214-1-Ig, Clone: 2H6C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68214-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68214-1-PBS cible CCT2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCT2 Protéine recombinante Ag19264
• Nom complet: chaperonin containing TCP1, subunit 2 (beta)
• Masse moléculaire calculée: 535 aa, 57 kDa
• Poids moléculaire observé: 50-58 kDa
• Numéro d’acquisition GenBank: BC113516
• Symbole du gène: CCT2
• Identification du gène (NCBI): 10576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with the molecular mass of 53 and 57 kDa. (PMID: 35699934)

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