Anticorps Polyclonal de lapin anti-CCT5

• Phare
• Validé par KD/KO

CCT5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 11603-1-AP

Synonymes

CCT epsilon, CCT5, CCTE, KIAA0098, TCP 1 epsilon

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Galerie de données de validation

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  WB analysis using 11603-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11603-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11603-1-AP

  WB result of CCT5 antibody (11603-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT5 transfected HEK-293 cells.

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  WB analysis of HeLa using 11603-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11603-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11603-1-AP

  IP Result of anti-CCT5 (IP:11603-1-AP, 4ug; Detection:11603-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human colon cancer using 11603-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11603-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11603-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11603-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11603-1-AP

  Immunofluorescent analysis of HepG2 cells, using CCT5 antibody 11603-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules A431, cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

11603-1-AP cible CCT5 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCT5 Protéine recombinante Ag2146
• Nom complet: chaperonin containing TCP1, subunit 5 (epsilon)
• Masse moléculaire calculée: 541 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC035499
• Symbole du gène: CCT5
• Identification du gène (NCBI): 22948
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.

Publications

Species	Application	Title
human	WB	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma ; KD Validated
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A
human	WB,IF	Proc Natl Acad Sci U S A; BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.; Authors - Seo Seongjin S
human	WB, IF	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
human,mouse	WB	Front Cell Dev Biol; TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis; Authors - Wen-Shuai Tang
chicken	WB,IF	Front Microbiol; Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.; Authors - Xiaohan Zhang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (01-03-2023)

Strong and clear band on the membrane.

• Applications: Western Blot