Anticorps Recombinant de lapin anti-CCT5

CCT5 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241510B3

N° de cat : 84310-2-RR

Synonymes

241510B3, CCT epsilon, CCTE, CCT-epsilon, Chaperonin containing T-complex polypeptide 1 subunit 5

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Galerie de données de validation

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  WB analysis using 84310-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84310-2-RR (CCT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 84310-2-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCT5 antibody (84310-2-RR, Clone: 241510B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84310-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84310-2-RR against Human CCT5 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules COLO 320, cellules HEK-293T, cellules HeLa, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84310-2-RR cible CCT5 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCT5 Protéine recombinante Ag2146
• Nom complet: chaperonin containing TCP1, subunit 5 (epsilon)
• Masse moléculaire calculée: 541 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC035499
• Symbole du gène: CCT5
• Identification du gène (NCBI): 22948
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.

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