Anticorps Polyclonal de lapin anti-CCT6A-Specific

• Phare
• Validé par KD/KO

CCT6A-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 19793-1-AP

Synonymes

CCT6A, Acute morphine dependence-related protein 2, CCT zeta, CCT zeta 1, CCT6

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Galerie de données de validation

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  WB analysis using 19793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 19793-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 19793-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 19793-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CCT6A-Specific antibody (19793-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules EC109, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

19793-1-AP cible CCT6A-Specific dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: chaperonin containing TCP1, subunit 6A (zeta 1)
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: NM_001762
• Symbole du gène: CCT6A
• Identification du gène (NCBI): 908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCT6A, also named as CCT6, CCTZ, CCT-zeta,Tcp20 and HTR3, belongs to the TCP-1 chaperonin family. CCT6A is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. CCT6A is known to play a role, in vitro, in the folding of actin and tubulin. The antibody has no cross reaction to CCT6B.

Publications

Species	Application	Title
human	WB,IF	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma
human	WB,IP	Adv Sci (Weinh); Exosomal CCT6A Secreted by Cancer-Associated Fibroblasts Interacts with β-Catenin to Enhance Chemoresistance and Tumorigenesis in Gastric Cancer; Authors - Hui Sun ; KD Validated
human	WB	Cancer Lett; Molecular chaperone CCT3 supports proper mitotic progression and cell proliferation in hepatocellular carcinoma cells.; Authors - Yuanyuan Zhang
human	WB	Mol Cell Proteomics; Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities.; Authors - Hussein Kaddour
human	WB,IHC	Oncol Lett; CCT6A is associated with CDC20, Enneking stage and prognosis in osteosarcoma, and its knockdown suppresses osteosarcoma cell viability and invasion; Authors - Ning Wang
mouse	IF	Signal Transduct Target Ther; Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease; Authors - Boyuan Ma

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