Anticorps Monoclonal anti-CCT8

• Phare
• Validé par KD/KO

CCT8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A7C3

N° de cat : 67539-1-PBS

Synonymes

C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta

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Galerie de données de validation

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  WB analysis using 67539-1-Ig (same clone as 67539-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67539-1-Ig (CCT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67539-1-Ig (same clone as 67539-1-PBS)

  WB result of CCT8 antibody (67539-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT8 transfected HEK-293 cells. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67539-1-Ig (same clone as 67539-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67539-1-Ig (CCT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67539-1-Ig (same clone as 67539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67539-1-Ig (CCT8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67539-1-Ig (same clone as 67539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67539-1-Ig (CCT8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67539-1-Ig (same clone as 67539-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CCT8 antibody (67539-1-Ig, Clone: 1A7C3 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67539-1-PBS cible CCT8 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCT8 Protéine recombinante Ag30082
• Nom complet: chaperonin containing TCP1, subunit 8 (theta)
• Masse moléculaire calculée: 548 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC012584
• Symbole du gène: CCT8
• Identification du gène (NCBI): 10694
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.

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