Anticorps Recombinant de lapin anti-Neprilysin/CD10

Neprilysin/CD10 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241322E1

N° de cat : 98116-1-RR

Synonymes

MME, Neprilysin, Enkephalinase, EC:3.4.24.11, CD10

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Galerie de données de validation

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  FC experiment of Reh using 98116-1-RR

  1x10^6 Reh cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone: 241322E1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

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  FC experiment of human peripheral blood leukocytes using 98116-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone: 241322E1) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98116-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Neprilysin/CD10 Rabbit Recombinant Antibody (98116-1-RR, Clone:241322E1)(red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98116-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98116-1-RR against Human CD10 were performed. The affinity constant is 9.9 nM.

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Applications testées

• Résultats positifs en cytométrie: human peripheral blood leukocytes, Reh cells

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98116-1-RR cible Neprilysin/CD10 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: membrane metallo-endopeptidase
• Masse moléculaire calculée: 750 aa, 86 kDa
• Numéro d’acquisition GenBank: BC101658
• Symbole du gène: CD10
• Identification du gène (NCBI): 4311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

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