Anticorps Monoclonal anti-CD100

• Phare
• Validé par KD/KO

CD100 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1C1A6

N° de cat : 66582-1-Ig

Synonymes

SEMA4D, 1C1A6, A8, CD 100, CD100 antigen

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Galerie de données de validation

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  WB analysis using 66582-1-Ig

  various cells were subjected to SDS PAGE followed by western blot with 66582-1-Ig (CD100 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66582-1-Ig

  WB result of CD100 antibody (66582-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD100 transfected HeLa cells.

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  IHC staining of human tonsillitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules K-562, cellules THP-1
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain, tissu de lymphome humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:225-1:900

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66582-1-Ig cible CD100 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD100 Protéine recombinante Ag26461
• Nom complet: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC054500
• Symbole du gène: SEMA4D/CD100
• Identification du gène (NCBI): 10507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

Publications

Species	Application	Title
human	WB	Oncol Lett; Low expression of SEMA4D as a potential predictive molecular marker of poor survival in patients with melanoma combined with liver cancer; Authors - Xiang Liu

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