Anticorps Recombinant de lapin anti-CD100

CD100 Recombinant Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241708D10

N° de cat : 84465-4-RR

Synonymes

SEMA4D, SEM4D, CD100 antigen, CD 100, A8

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Galerie de données de validation

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  IF Staining of Jurkat using 84465-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD100 antibody (84465-4-RR, Clone: 241708D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84465-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84465-4-RR against Human CD100 were performed. The affinity constant is 3.21 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84465-4-RR cible CD100 dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Masse moléculaire calculée: 96 kDa
• Numéro d’acquisition GenBank: BC054500
• Symbole du gène: SEMA4D/CD100
• Identification du gène (NCBI): 10507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

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