Anticorps Monoclonal anti-CD107a / LAMP1
CD107a / LAMP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra)
Hôte / Isotype
Mouse / IgG1, kappa
Réactivité testée
Humain et plus (2)
Applications
WB, IF/ICC, FC (Intra)
Conjugaison
Non conjugué
CloneNo.
H4A3
N° de cat : 65051-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HEK-293, cellules HeLa, cellules Jurkat |
Résultats positifs en IF/ICC | cellules HeLa, |
Résultats positifs en FC (Intra) | cellules HeLa |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 3 publications below |
IF | See 8 publications below |
Informations sur le produit
65051-1-Ig cible CD107a / LAMP1 dans les applications de WB, IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | Humain, singe, souris |
Hôte / Isotype | Mouse / IgG1, kappa |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | Cellules adhérentes humaines de sang périphérique |
Nom complet | lysosomal-associated membrane protein 1 |
Masse moléculaire calculée | 45 kDa |
Poids moléculaire observé | 100-120 kDa |
Numéro d’acquisition GenBank | BC006345 |
Symbole du gène | LAMP1 |
Identification du gène (NCBI) | 3916 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine G |
Tampon de stockage | PBS with 0.09% sodium azide |
Conditions de stockage | Store at 2-8°C. Stable for one year after shipment. |
Informations générales
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protocole
Product Specific Protocols | |
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WB protocol for CD107a / LAMP1 antibody 65051-1-Ig | Download protocol |
IF protocol for CD107a / LAMP1 antibody 65051-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Eur J Pharmacol Cycloheptylprodigiosin from marine bacterium Spartinivicinus ruber MCCC 1K03745T induces a novel form of cell death characterized by Golgi disruption and enhanced secretion of cathepsin D in Non-small cell lung cancer cell lines | ||
Autophagy ATP6V1D drives hepatocellular carcinoma stemness and progression via both lysosome acidification-dependent and -independent mechanisms | ||
Cell Rep A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma | ||
MAbs Targeted protein degradation through site-specific antibody conjugation with mannose 6-phosphate glycan | ||
CNS Neurosci Ther Inhibition of Salt-Inducible Kinase 2 Protects Motor Neurons From Degeneration in ALS by Activating Autophagic Flux and Enhancing mTORC1 Activity |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Parijat (Verified Customer) (09-23-2025) | Works well for IF. Typical lysosomal pattern was observed.
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FH Christine (Verified Customer) (10-02-2024) | I randomly tried this antibody for western blotting (because this clone has been shown to work in WB in publications) and it worked beautifully at 1:500 on total lysates of HEK293T cells. Could be diluted much further.
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