Anticorps Monoclonal anti-CD107a / LAMP1

CD107a / LAMP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain et plus (2)

Applications

WB, IF/ICC, FC (Intra)

Conjugaison

Non conjugué

Publications(11)

CloneNo.

H4A3

N° de cat : 65051-1-Ig

Synonymes

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3



Applications testées

Résultats positifs en WBcellules HEK-293, cellules HeLa, cellules Jurkat
Résultats positifs en IF/ICCcellules HeLa,
Résultats positifs en FC (Intra)cellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65051-1-Ig cible CD107a / LAMP1 dans les applications de WB, IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain, singe, souris
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Cellules adhérentes humaines de sang périphérique
Nom complet lysosomal-associated membrane protein 1
Masse moléculaire calculée45 kDa
Poids moléculaire observé100-120 kDa
Numéro d’acquisition GenBankBC006345
Symbole du gène LAMP1
Identification du gène (NCBI) 3916
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 0.09% sodium azide
Conditions de stockageStore at 2-8°C. Stable for one year after shipment.

Informations générales

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protocole

Product Specific Protocols
WB protocol for CD107a / LAMP1 antibody 65051-1-IgDownload protocol
IF protocol for CD107a / LAMP1 antibody 65051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,monkeyWB,IF

Autophagy

Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress

Authors - Jia-min Yan
humanIF

Eur J Pharmacol

Cycloheptylprodigiosin from marine bacterium Spartinivicinus ruber MCCC 1K03745T induces a novel form of cell death characterized by Golgi disruption and enhanced secretion of cathepsin D in Non-small cell lung cancer cell lines

Authors - Xiaosi Lin
humanIF

Autophagy

ATP6V1D drives hepatocellular carcinoma stemness and progression via both lysosome acidification-dependent and -independent mechanisms

Authors - Zhijie Xu
humanIF

Cell Rep

A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma

Authors - Hongli Zhang
humanIF

MAbs

Targeted protein degradation through site-specific antibody conjugation with mannose 6-phosphate glycan

Authors - Kaori Mukai
mouseIF

CNS Neurosci Ther

Inhibition of Salt-Inducible Kinase 2 Protects Motor Neurons From Degeneration in ALS by Activating Autophagic Flux and Enhancing mTORC1 Activity

Authors - Weiwei Liang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Parijat (Verified Customer) (09-23-2025)

Works well for IF. Typical lysosomal pattern was observed.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mammalian Cells
FH

Christine (Verified Customer) (10-02-2024)

I randomly tried this antibody for western blotting (because this clone has been shown to work in WB in publications) and it worked beautifully at 1:500 on total lysates of HEK293T cells. Could be diluted much further.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293T
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