Anticorps Polyclonal de lapin anti-CD107a / LAMP1

CD107a / LAMP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 33243-1-AP

Synonymes

CD107a, Lamp1, Lamp 1

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Galerie de données de validation

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  WB analysis using 33243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33243-1-AP (CD107a / LAMP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 33243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 33243-1-AP (CD107a / LAMP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 33243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 33243-1-AP (CD107a / LAMP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 33243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33243-1-AP (CD107a / LAMP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 33243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33243-1-AP (CD107a / LAMP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 33243-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CD107a / LAMP1 antibody (33243-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: J774A.1 cells, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu hépatique de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

33243-1-AP cible CD107a / LAMP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD107a / LAMP1 Protéine recombinante Eg3858
• Nom complet: lysosomal-associated membrane protein 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: NM_010684.3
• Symbole du gène: CD107a
• Identification du gène (NCBI): 16783
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

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