Anticorps Recombinant de lapin anti-CD107a / LAMP1

CD107a / LAMP1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242116E8

N° de cat : 84658-5-PBS

Synonymes

CD107a, LAMP1, 242116E8, CD107 antigen-like family member A, CD107a/LAMP1

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Galerie de données de validation

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  WB analysis using 84658-5-RR (same clone as 84658-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84658-5-RR (CD107a / LAMP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84658-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 84658-5-RR (same clone as 84658-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84658-5-RR (CD107a / LAMP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84658-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 84658-5-RR (same clone as 84658-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84658-5-RR (CD107a / LAMP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84658-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84658-5-RR (same clone as 84658-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD107a / LAMP1 antibody (84658-5-RR, Clone: 242116E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84658-5-PBS in a different storage buffer formulation.

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Informations sur le produit

84658-5-PBS cible CD107a / LAMP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD107a / LAMP1 Protéine recombinante Eg2611
• Nom complet: lysosomal-associated membrane protein 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: NM_005561.4
• Symbole du gène: LAMP1
• Identification du gène (NCBI): 3916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

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