Anticorps Recombinant de lapin anti-CD107a / LAMP1

CD107a / LAMP1 Recombinant Antibody for IF/ICC, FC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC, FC (Intra)

Conjugaison

Non conjugué

CloneNo.

242116E8

N° de cat : 98266-2-RR

Synonymes

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, LAMP 1

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Galerie de données de validation

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  IF Staining of HepG2 using 98266-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LAMP1 antibody (98266-2-RR, Clone: 242116E8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 98266-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CD107a / LAMP1 antibody (98266-2-RR, Clone: 242116E8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of Jurkat using 98266-2-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human CD107a / LAMP1 Rabbit RecAb (98266-2-RR, Clone: 242116E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of human peripheral blood platelets using 98266-2-RR

  1x10^6 thrombin-treated human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD107a / LAMP1 Rabbit RecAb (98266-2-RR, Clone: 242116E8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). 1x10^6 untreated human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD107a / LAMP1 Rabbit RecAb (98266-2-RR, Clone:242116E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules Jurkat,
• Résultats positifs en cytométrie: Thrombin treated human peripheral blood platelets,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

98266-2-RR cible CD107a / LAMP1 dans les applications de IF/ICC, FC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD107a / LAMP1 Protéine recombinante Eg2611
• Nom complet: lysosomal-associated membrane protein 1
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: NM_005561.4
• Symbole du gène: LAMP1
• Identification du gène (NCBI): 3916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

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