Anticorps Multi-Recombinant de lapin anti-CD107a / LAMP1

CD107a / LAMP1 Multi-Recombinant Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : RMX00025

Synonymes

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, LAMP 1

---

Galerie de données de validation

• 

  WB analysis using RMX00025

  Untreated and PNGaseF-treated HeLa cell lysates were subjected to SDS PAGE followed by western blot with RMX00025 (CD107a / LAMP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using RMX00025

  IP result of anti-CD107a / LAMP1 (IP:RMX00025, 4ug; Detection:RMX00025 1:20000) with HeLa cells lysate 1200 ug.

• 

  IF Staining of HepG2 using RMX00025

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CD107a / LAMP1 antibody (RMX00025) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

RMX00025 cible CD107a / LAMP1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Multi-Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: lysosomal-associated membrane protein 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 40-48 kDa, 90-120 kDa
• Numéro d’acquisition GenBank: NM_005561.4
• Symbole du gène: LAMP1
• Identification du gène (NCBI): 3916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: N/A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

{{ptg:RelatedPrimaryAntibodies}}