Anticorps Polyclonal de lapin anti-CD109

CD109 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP,ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 18715-1-AP

Synonymes

CD109, CD109 antigen, CD109 molecule, CPAMD7, p180, Platelet specific Gov antigen, r150, RP11 525G3.1

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Galerie de données de validation

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  WB analysis of PC-3 using 18715-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18715-1-AP (CD109 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 18715-1-AP

  IP Result of anti-CD109 (IP:18715-1-AP, 4ug; Detection:18715-1-AP 1:600) with PC-3 cells lysate 5000ug.

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Applications testées

• Résultats positifs en WB: cellules PC-3,
• Résultats positifs en IP: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

18715-1-AP cible CD109 dans les applications de WB, IP,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: CD109 molecule
• Masse moléculaire calculée: 162 kDa
• Numéro d’acquisition GenBank: BC152996
• Symbole du gène: CD109
• Identification du gène (NCBI): 135228
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD109, also named as CPAMD7, is a member of the alpha2-macroglobulin/complement superfamily. It is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein of approximately 170kd which is found on the cell surface of platelets, activated T-cells, and endothelial cells. CD109 binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta). CD109 has been identified as part of the TGF-beta receptor system in human keratinocytes. Up regulation of CD109 expression has been observed in several different types of tumor.CD109 is a glycosyl-phosphatidylinositol-anchored glycoprotein that is a member of the a2-macroglobulin/C3, C4, C5 family of thioester-containing proteins. There're 2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. The antibody can recognize all these 4 isoforms.

Publications

Species	Application	Title
human	WB	Oncol Lett; CD109 expression is upregulated in penile squamous cell carcinoma.; Authors - Fengyun Dong