Anticorps Monoclonal anti-CD117 (c-Kit)

CD117 (c-Kit) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

ACK2

N° de cat : CL488-65094

Synonymes

Bs, c KIT, CD117, CD117 (c-Kit), Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, kit oncogene, SCO1, SCO5, SOW3, Ssm, Tr kit



Applications testées

Résultats positifs en cytométriecellules médullaires de souris,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65094 cible CD117 (c-Kit) dans les applications de FC et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Rat / IgG2b, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Mastocytes dépendant de l'IL-3 de souris
Nom complet kit oncogene
Numéro d’acquisition GenBankBC052457
Symbole du gène Kit
Identification du gène (NCBI) 16590
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

Protocole

Product Specific Protocols
FC protocol for CL Plus 488 CD117 (c-Kit) antibody CL488-65094Download protocol
Standard Protocols
Click here to view our Standard Protocols