Anticorps Polyclonal de lapin anti-CD117/c-Kit

CD117/c-Kit Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 31337-1-AP

Synonymes

Kit, CD117, c KIT

---

Galerie de données de validation

• 

  WB analysis using 31337-1-AP

  MC/9 cells were subjected to SDS PAGE followed by western blot with 31337-1-AP (CD117/c-Kit antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31337-1-AP

  Mouse testis tissue was subjected to SDS PAGE followed by western blot with 31337-1-AP (CD117/c-kit antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules MC/9, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31337-1-AP cible CD117/c-Kit dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: kit oncogene
• Masse moléculaire calculée: 109 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_001122733.1
• Symbole du gène: CD117
• Identification du gène (NCBI): 16590
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

{{ptg:RelatedPrimaryAntibodies}}