Anticorps Recombinant de lapin anti-CD117/c-Kit

CD117/c-Kit Recombinant Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242068G5

N° de cat : 84259-10-RR

Synonymes

CD117, KIT, C Kit, c-Kit, EC:2.7.10.1

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Galerie de données de validation

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  IHC staining of human colon using 84259-10-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84259-10-RR (C-Kit antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human colon using 84259-10-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84259-10-RR (C-Kit antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of Human Kidney(renal cell) Cancer using 84259-10-RR

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 84259-10-RR (C-Kit antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Kidney(renal cell) Cancer using 84259-10-RR

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 84259-10-RR (C-Kit antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Gastrointestinal Stromal Tumor using 84259-10-RR

  Immunohistochemical analysis of paraffin-embedded Gastrointestinal Stromal Tumor tissue slide using 84259-10-RR (C-Kit antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Gastrointestinal Stromal Tumor using 84259-10-RR

  Immunohistochemical analysis of paraffin-embedded Gastrointestinal Stromal Tumor tissue slide using 84259-10-RR (C-Kit antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de côlon humain, human gastrointestinal stromal tumor tissue, human kidney (renal cell) cancer; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84259-10-RR cible CD117/c-Kit dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Masse moléculaire calculée: 976 aa, 110 kDa
• Numéro d’acquisition GenBank: BC071593
• Symbole du gène: CD117
• Identification du gène (NCBI): 3815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

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