Anticorps Recombinant de lapin anti-CD117/c-Kit

CD117/c-Kit Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241313C5

N° de cat : 98377-1-RR

Synonymes

CD117, KIT, C Kit, c-Kit, EC:2.7.10.1

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Galerie de données de validation

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  FC experiment of TF-1 using 98377-1-RR

  1x10^6 TF-1 cells were surface stained with 0.25 ug Anti-Human C-Kit Rabbit RecAb (98377-1-RR, Clone:241313C5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed.

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  FC experiment of human blood cells using 98377-1-RR

  1x10^6 human blood cells were surface stained with 0.25 ug Anti-Human C-Kit Rabbit RecAb (98377-1-RR, Clone:241313C5) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD34. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: cellules TF-1, FC receptor blocked human blood cells

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98377-1-RR cible CD117/c-Kit dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Masse moléculaire calculée: 976 aa, 110 kDa
• Numéro d’acquisition GenBank: BC071593
• Symbole du gène: CD117
• Identification du gène (NCBI): 3815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

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