Anticorps Monoclonal anti-CD11b

CD11b Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(56)

CloneNo.

1C7C2

Avis (2)

N° de cat : 66519-1-Ig

Synonymes

1C7C2, CD11 antigen-like family member B, Cell surface glycoprotein MAC-1 subunit alpha, CR 3 alpha chain, CR-3 alpha chain

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Galerie de données de validation

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  WB analysis of TF-1 using 66519-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human bone marrow using 66519-1-Ig

  human bone marrow tissue were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66519-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66519-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66519-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66519-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD11B/Integrin Alpha M antibody (66519-1-Ig, Clone: 1C7C2 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules TF-1, tissu de moelle osseuse humain
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 7 publications below
• IF: See 27 publications below

Informations sur le produit

66519-1-Ig cible CD11b dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: integrin, alpha M (complement component 3 receptor 3 subunit)
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: NM_000632
• Symbole du gène: CD11b
• Identification du gène (NCBI): 3684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
		Cell Rep Med; Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity; Authors - Xingyu Zhou
	IF	J Exp Med; TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.; Authors - Dehong Yan
human	IF	Cell Rep; Inflammatory dendritic cells restrain CD11b+CD4+ CTLs via CD200R in human NSCLC; Authors - Mingjie Lin
		Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang
	IF	Apoptosis; ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments; Authors - Qian Yan
	IF	Life Sci; Urine-derived stem cells from patients alleviate lupus nephritis via regulating macrophage polarization in a CXCL14-dependent manner; Authors - Yunfei Xia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (09-05-2024)

CD11b did not work as good as expected, not showing my microglia, but rather appearing in some small neurons, and most of the marking seems to be autofluorescence from the human brain tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

CD11b Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:66519-1-Ig)

FH

Santhosh (Verified Customer) (04-15-2019)

Human FFPE tonsil tissue, HIER pH6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200

CD11b Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66519-1-Ig)

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