Anticorps Recombinant de lapin anti-CD11a

CD11a Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

TS2/4

N° de cat : 65518-1-PBS

Synonymes

CD11 antigen-like family member A, Integrin alpha L, Integrin alpha-L, ITGAL, Leukocyte adhesion glycoprotein LFA-1 alpha chain

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Galerie de données de validation

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  FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65518-1-PBS

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65518-1-PBS

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65518-1-PBS

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS Clone:TS2/4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65518-1-PBS cible CD11a dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lignée de CTL HLA-DR
• Nom complet: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Masse moléculaire calculée: 129 kDa
• Numéro d’acquisition GenBank: BC008777
• Symbole du gène: CD11a
• Identification du gène (NCBI): 3683
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. Stable for one year after shipment.

Informations générales

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

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