Anticorps Monoclonal anti-CD11a

CD11a Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

CloneNo.

TS2/4

N° de cat : CL488-65194

Synonymes

CD11A, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using CL488-65194

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD11a (CL488-65194, Clone:TS2/4), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL488-65194

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD11a (CL488-65194, Clone:TS2/4), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL488-65194 cible CD11a dans les applications de IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de CTL HLA-DR
• Nom complet: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Masse moléculaire calculée: 129 kDa
• Numéro d’acquisition GenBank: BC008777
• Symbole du gène: CD11a
• Identification du gène (NCBI): 3683
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

Publications

Species	Application	Title
human	IF	Nat Commun; The autophagy component LC3 regulates lymphocyte adhesion via LFA1 transport in response to outside-in signaling; Authors - Naoyuki Kondo

{{ptg:RelatedPrimaryAntibodies}}