Anticorps Recombinant de lapin anti-CD11a

CD11a Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

TS2/4

N° de cat : FITC-65518

Synonymes

CD11 antigen-like family member A, Integrin alpha L, Integrin alpha-L, ITGAL, Leukocyte adhesion glycoprotein LFA-1 alpha chain

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-65518

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11a Rabbit Recombinant Antibody (FITC-65518, Clone:TS2/4) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65518

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11a Rabbit Recombinant Antibody (FITC-65518, Clone:TS2/4) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65518

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11a Rabbit Recombinant Antibody (FITC-65518, Clone:TS2/4) (left) or 5 ul FITC Plus Anti-Human CD11a Mouse Monoclonal Antibody (FITC-65194, Clone:TS2/4) (right). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65518 cible CD11a dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lignée de CTL HLA-DR
• Nom complet: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Masse moléculaire calculée: 129 kDa
• Numéro d’acquisition GenBank: BC008777
• Symbole du gène: CD11a
• Identification du gène (NCBI): 3683
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

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