Anticorps Monoclonal anti-CD11a/Integrin Alpha L

CD11a/Integrin Alpha L Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1E3H12

N° de cat : 66256-1-PBS

Synonymes

CD11a, 1E3H12, CD11 antigen-like family member A, Integrin alpha L, Integrin alpha-L

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Galerie de données de validation

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  WB analysis of Ramos using 66256-1-Ig (same clone as 66256-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66256-1-Ig (CD11a/Integrin Alpha L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 66256-1-Ig (same clone as 66256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66256-1-PBS cible CD11a/Integrin Alpha L dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD11a/Integrin Alpha L Protéine recombinante Ag7813
• Nom complet: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Masse moléculaire calculée: 129 kDa
• Numéro d’acquisition GenBank: BC008777
• Symbole du gène: CD11a
• Identification du gène (NCBI): 3683
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

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