Anticorps Monoclonal anti-CD11b

CD11b Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

rat

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

Publications(3)

CloneNo.

OX42

N° de cat : FITC-65229

Synonymes

integrin alpha M, Itgam, OX42

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Galerie de données de validation

• 

  FC experiment of rat bone marrow cells using FITC-65229

  1x10^6 rat bone marrow cells were surface stained with 0.25 ug FITC Plus Anti-Rat CD11b/c (OX42) (FITC-65229, Clone: OX42) or 0.25 ug FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: rat bone marrow cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 3 publications below

Informations sur le produit

FITC-65229 cible CD11b dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Rat peritoneal macrophages
• Nom complet: integrin alpha M
• Masse moléculaire calculée: 127 kDa
• Numéro d’acquisition GenBank: NM_012711
• Symbole du gène: CD11b
• Identification du gène (NCBI): 25021
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).

Publications

Species	Application	Title
rat	FC	Mol Med Rep; Discovery of microglia gonadotropin‑releasing hormone receptor and its potential role in polycystic ovarian syndrome; Authors - Fan Wang
rat	FC	Biomater Res; CD62E- and ROS-Responsive ETS Improves Cartilage Repair by Inhibiting Endothelial Cell Activation through OPA1-Mediated Mitochondrial Homeostasis; Authors - Pengcheng Tu
	FC	Heliyon; Peimine promotes microglial polarization to the M2 phenotype to attenuate drug-resistant epilepsy through suppressing the TLR4/NF-κB/HIF-1α signaling pathway in a rat model and in BV-2 microglia; Authors - Chongchong Liu

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