Anticorps Recombinant de lapin anti-CD122/IL-2RB

CD122/IL-2RB Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241677H7

N° de cat : 98151-1-PBS

Synonymes

IL2RB, IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta

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Galerie de données de validation

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  FC experiment of human PBMCs using 98151-1-RR (same clone as 98151-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD122/IL-2RB Rabbit Recombinant Antibody (98151-1-RR, Clone: 241677H7) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56 Rabbit Recombinant Antibody (CL647-98033, Clone: 240078A7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98151-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98151-1-RR (same clone as 98151-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD122/IL-2RB Rabbit Recombinant Antibody (98151-1-RR, Clone: 241677H7) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98151-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98151-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98151-1-RR against Human CD122/IL-2RB were performed. The affinity constant is 0.645 nM.

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Informations sur le produit

98151-1-PBS cible CD122/IL-2RB dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 2 receptor, beta
• Masse moléculaire calculée: 551 aa, 61 kDa
• Numéro d’acquisition GenBank: BC025691
• Symbole du gène: IL2RB
• Identification du gène (NCBI): 3560
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to the ability to bind interleukin 2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor-mediated endocytosis and transduces the mitogenic signals of interleukin 2.

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