Anticorps Monoclonal anti-CD123

CD123 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HI12H7

N° de cat : CL488-65256

Synonymes

CD123, hIL 3Ra, IL 3 receptor subunit alpha, IL 3R alpha, IL 3R subunit alpha, IL 3RA, IL3R, IL3RA, IL3RAY, IL3RX, IL3RY



Applications testées

Résultats positifs en cytométrieCMSP humaines,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65256 cible CD123 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet interleukin 3 receptor, alpha (low affinity)
Masse moléculaire calculée 378 aa, 43 kDa
Numéro d’acquisition GenBankBC035407
Symbole du gène IL3RA
Identification du gène (NCBI) 3563
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD123, also referred to as interleukin-3 receptor subunit alpha (IL-3Rα), belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). CD123 is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to CD123 and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.

Protocole

Product Specific Protocols
FC protocol for CL Plus 488 CD123 antibody CL488-65256Download protocol
Standard Protocols
Click here to view our Standard Protocols