Anticorps Polyclonal de lapin anti-CD126/IL-6R alpha

• Phare
• Validé par KD/KO

CD126/IL-6R alpha Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23457-1-PBS

Synonymes

CD126, IL-6R alpha, gp80, IL 6 receptor subunit alpha, IL 6R 1

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Galerie de données de validation

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  WB analysis using 23457-1-AP (same clone as 23457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 23457-1-AP (same clone as 23457-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

  IP result of anti-CD126/IL-6R alpha (IP:23457-1-AP, 4ug; Detection:23457-1-AP 1:3000) with mouse spleen tissue lysate 2480 ug. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23457-1-PBS cible CD126/IL-6R alpha dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD126/IL-6R alpha Protéine recombinante Ag18263
• Nom complet: interleukin 6 receptor
• Masse moléculaire calculée: 468 aa, 52 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC132684
• Symbole du gène: IL-6R alpha
• Identification du gène (NCBI): 3570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).

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