Anticorps Recombinant de lapin anti-CD126/IL-6R alpha

CD126/IL-6R alpha Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240734A7

N° de cat : 98057-1-RR

Synonymes

CD126, IL-6R alpha, 240734A7, IL 6R alpha, IL 6R 1

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Galerie de données de validation

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  FC experiment of human PBMCs using 98057-1-RR

  1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3) and 0.25 ug Anti-Human CD126/IL-6R alpha Rabbit Recombinant Antibody (98057-1-RR, Clone: 240734A7), or Isotype Control. Cells were not fixed.

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  FC experiment of human PBMCs using 98057-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD126/IL-6R alpha Rabbit Recombinant Antibody (98057-1-RR, Clone:240734A7) (red), or Isotype Control (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98057-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98057-1-RR against Human CD126/IL-6R alpha were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98057-1-RR cible CD126/IL-6R alpha dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 6 receptor
• Masse moléculaire calculée: 468 aa, 52 kDa
• Numéro d’acquisition GenBank: BC132684
• Symbole du gène: IL-6R alpha
• Identification du gène (NCBI): 3570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).

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