Anticorps Recombinant de lapin anti-CD126/IL-6R alpha

CD126/IL-6R alpha Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240734A7

N° de cat : CL647-98057

Synonymes

CD126, IL-6R alpha, 240734A7, gp80, IL 6 receptor subunit alpha

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Galerie de données de validation

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  FC experiment of human PBMCs using CL647-98057

  1x10^6 human PBMCs were surface stained with PE Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (PE-65569, Clone: OKT3), and 5 ul CoraLite® Plus 647 Anti-Human CD126/IL-6R alpha Rabbit RecAb (CL647-98057, Clone:240734A7) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-98057

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD126/IL-6R alpha Rabbit RecAb (CL647-98057, Clone: 240734A7) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98057 cible CD126/IL-6R alpha dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD126/IL-6R alpha Protéine recombinante Eg0490
• Nom complet: interleukin 6 receptor
• Masse moléculaire calculée: 468 aa, 52 kDa
• Numéro d’acquisition GenBank: BC132684
• Symbole du gène: IL-6R alpha
• Identification du gène (NCBI): 3570
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).

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