Anticorps Monoclonal anti-CD127 (IL-7Ra)

CD127 (IL-7Ra) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a

Réactivité testée

souris

Applications

FC

Conjugaison

PE Fluorescent Dye

Publications

1

CloneNo.

A7R34

N° de cat : PE-65093

Synonymes

CD127, IL 7Ralpha, IL- 7Ralpha, Il7r, IL-7Ra, interleukin 7 receptor

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Galerie de données de validation

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  FC experiment of mouse splenocytes using PE-65093

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug APC-Anti-Mouse CD3 (APC-65077, clone 17A2) and 0.25 ug PE-Anti-Mouse CD127 (IL-7Ra) (PE-65093, clone A7R34). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-65093

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug APC-Anti-Mouse CD3 (APC-65077, clone 17A2) and 0.25 ug PE-rat IgG2a isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

PE-65093 cible CD127 (IL-7Ra) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine de fusion IL-7Ra-IgG1
• Nom complet: interleukin 7 receptor
• Numéro d’acquisition GenBank: BC089571
• Symbole du gène: Il7r
• Identification du gène (NCBI): 16197
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Stocker à une température comprise entre 2 °C et 8 °C. Éviter toute exposition à la lumière.

Informations générales

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).

Publications

Species	Application	Title
mouse	FC	J Inflamm Res; Sodium Butyrate Ameliorates Type 2 Diabetes-Related Sarcopenia Through IL-33-Independent ILC2s/IL-13/STAT3 Signaling Pathway; Authors - Yuan Cao