Anticorps Monoclonal anti-CD13

CD13 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain, primates non humains

Applications

IF/ICC, FC

Conjugaison

Non conjugué

CloneNo.

WM15

N° de cat : 65186-1-PBS

Synonymes

APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M

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Galerie de données de validation

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  IF Staining of HUVEC using 65186-1-Ig (same clone as 65186-1-PBS)

  Immunofluorescent analysis of un-fixed HUVEC cells using CD13 antibody (65186-1-Ig, Clone: WM15 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 65186-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 65186-1-Ig (same clone as 65186-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (65186-1-Ig, Clone: WM15 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 65186-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65186-1-Ig (same clone as 65186-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD13 (65186-1-Ig, Clone:WM15) and FITC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65186-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65186-1-Ig (same clone as 65186-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD13 (65186-1-Ig, Clone:WM15) and FITC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65186-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65186-1-PBS cible CD13 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons Humain, primates non humains

• Réactivité: Humain, primates non humains
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules AML humaines
• Nom complet: alanyl (membrane) aminopeptidase
• Masse moléculaire calculée: 110 kDa
• Numéro d’acquisition GenBank: BC058928
• Symbole du gène: CD13
• Identification du gène (NCBI): 290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

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