Anticorps Recombinant de lapin anti-CD13

CD13 Recombinant Antibody for IF/ICC, FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF/ICC, FC

Conjugaison

Non conjugué

CloneNo.

WM15

N° de cat : 65633-1-MR

Synonymes

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP-M

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Galerie de données de validation

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  IF Staining of THP-1 using 65633-1-MR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (65633-1-MR, Clone: WM15 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of human PBMCs using 65633-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD13 (WM15) Mouse IgG2a RecAb (65633-1-MR, Clone:WM15) and PE-conjugated Goat Anti-Mouse IgG, or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of human PBMCs using 65633-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD13 (WM15) Mouse IgG2a RecAb (65633-1-MR, Clone: WM15) (red) and PE-conjugated Goat Anti-Mouse IgG, or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: cellules THP-1,
• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

65633-1-MR cible CD13 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cellules AML humaines
• Nom complet: alanyl (membrane) aminopeptidase
• Masse moléculaire calculée: 110 kDa
• Numéro d’acquisition GenBank: BC058928
• Symbole du gène: CD13
• Identification du gène (NCBI): 290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane protein expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

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