Anticorps Recombinant de lapin anti-CD13

CD13 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251501F8

N° de cat : 86620-3-RR

Synonymes

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP-M

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Galerie de données de validation

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  WB analysis using 86620-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86620-3-RR (CD13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PANC-1 using 86620-3-RR

  PANC-1 cells were subjected to SDS PAGE followed by western blot with 86620-3-RR (CD13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 86620-3-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (86620-3-RR, Clone: 251501F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: A-204 cells, tissu rénal de souris
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86620-3-RR cible CD13 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD13 Protéine recombinante Eg3748
• Nom complet: alanyl (membrane) aminopeptidase
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC058928
• Symbole du gène: CD13
• Identification du gène (NCBI): 290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

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