Anticorps Monoclonal anti-CD133

CD133 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(64)

CloneNo.

2B8A2

Avis (1)

N° de cat : 66666-1-Ig

Synonymes

2B8A2, AC133, Antigen AC133, CORD12, MCDR2

---

Galerie de données de validation

• 

  WB analysis of HT-29 using 66666-1-Ig

  Untreated and PNGase F-treated lysates of HT-29 cells and Caco-2 cells were subjected to SDS PAGE followed by western blot with 66666-1-Ig (CD133 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  IHC staining of human kidney using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66666-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse colon using 66666-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD133 antibody (66666-1-Ig, Clone: 2B8A2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HT-29, cellules Caco-2
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 40 publications below
• IHC: See 16 publications below
• IF: See 20 publications below
• ELISA: See 1 publications below

Informations sur le produit

66666-1-Ig cible CD133 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD133 Protéine recombinante Ag13327
• Nom complet: prominin 1
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 115 kDa, 80-90 kDa
• Numéro d’acquisition GenBank: BC012089
• Symbole du gène: CD133
• Identification du gène (NCBI): 8842
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).

Publications

Species	Application	Title
human	WB,IF	J Exp Clin Cancer Res; ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma; Authors - Tong Xu
human	WB	Int J Cancer; Metabolic characterization of sphere-derived prostate cancer stem cells reveals aberrant urea cycle in stemness maintenance; Authors - Yuanyuan Luo
human	IHC	Mol Ther Oncolytics; ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer.; Authors - Hengqiang Zhao
human	WB,IHC,IF	Aging (Albany NY); The critical role of peroxiredoxin-2 in colon cancer stem cells.; Authors - Linglong Peng
human	IF	Pharmacol Res; Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B.; Authors - Xiaomei Li
human	IHC	Int J Mol Sci; Promoter Methylation of Cancer Stem Cell Surface Markers as an Epigenetic Biomarker for Prognosis of Oral Squamous Cell Carcinoma; Authors - Yu Kyeong Han

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:1000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cells

{{ptg:RelatedPrimaryAntibodies}}