Anticorps Polyclonal de lapin anti-CD138/Syndecan-1

• Phare
• Validé par KD/KO

CD138/Syndecan-1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10593-1-PBS

Synonymes

CD138, SDC, SDC1, SYND1, syndecan

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Galerie de données de validation

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  WB analysis using 10593-1-AP (same clone as 10593-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10593-1-AP (same clone as 10593-1-PBS)

  WB result of CD138/Syndecan-1 antibody (10593-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD138/Syndecan-1 transfected HeLa cells. This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10593-1-AP (same clone as 10593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10593-1-AP (same clone as 10593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 10593-1-AP (same clone as 10593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10593-1-PBS cible CD138/Syndecan-1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD138/Syndecan-1 Protéine recombinante Ag0823
• Nom complet: syndecan 1
• Masse moléculaire calculée: 32.5 kDa
• Poids moléculaire observé: 60-100 kDa, 130 kDa
• Numéro d’acquisition GenBank: BC008765
• Symbole du gène: CD138
• Identification du gène (NCBI): 6382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.

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