Anticorps Recombinant de lapin anti-CD138/Syndecan-1

CD138/Syndecan-1 Recombinant Antibody for IHC, FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, FC

Conjugaison

Non conjugué

CloneNo.

240422C4

N° de cat : 98051-1-RR

Synonymes

CD138, 240422C4, syndecan, SYND1, SDC1

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Galerie de données de validation

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  IHC staining of human tonsillitis using 98051-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98051-1-RR (CD138/Syndecan-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 98051-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98051-1-RR (CD138/Syndecan-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of U266 using 98051-1-RR

  1x10^6 U266 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody (98051-1-RR, Clone: 240422C4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98051-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98051-1-RR against Human CD138/Syndecan-1 were performed. The affinity constant is 20.8 nM.

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: U266 cells,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

98051-1-RR cible CD138/Syndecan-1 dans les applications de IHC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: syndecan 1
• Masse moléculaire calculée: 32.5 kDa
• Numéro d’acquisition GenBank: BC008765
• Symbole du gène: CD138
• Identification du gène (NCBI): 6382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

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