Anticorps Monoclonal anti-CD14

CD14 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

13

CloneNo.

2C1D9

Avis (3)

N° de cat : 60253-1-Ig

Synonymes

CD14, CD14 molecule

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Galerie de données de validation

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  WB analysis of THP-1 using 60253-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60253-1-Ig

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60253-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60253-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of RAW 264.7 using 60253-1-Ig

  Immunofluorescent analysis of RAW 264.7 cells using 60253-1-Ig (CD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

• 

  IF Staining of human tonsillitis using 60253-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD14 antibody (60253-1-Ig, Clone: 2C1D9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of THP-1 using 60253-1-Ig

  1X10^6 THP-1 cells were surface stained with 0.4 ug Anti-Human CD14 (60253-1-Ig, Clone:2C1D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules RAW 264.7
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules RAW 264.7, tissu d'amygdalite humain
• Résultats positifs en cytométrie: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

60253-1-Ig cible CD14 dans les applications de WB, IHC, IF, FC, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, Lapin, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD14 Protéine recombinante Ag10693
• Nom complet: CD14 molecule
• Masse moléculaire calculée: 375 aa, 40 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC010507
• Symbole du gène: CD14
• Identification du gène (NCBI): 929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Publications

Species	Application	Title
human	IHC	Mol Cell; Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.; Authors - Jia Xiong
human	IF	Arthritis Rheumatol; Attenuation of rheumatoid arthritis through the inhibition of caspase3/GSDME-mediated pyroptosis induced by TNF-α.; Authors - Zeqing Zhai
rabbit	IF	Bioact Mater; Novel magnetic silk fibroin scaffolds with delayed degradation for potential long-distance vascular repair.; Authors - Xin Liu
mouse	WB,IF	Sci Rep; High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage.; Authors - Jianhua Peng
mouse	Cell treatment	Front Pharmacol; Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway; Authors - Sairong Fan
human	IHC	J Cancer; MMP9 and IGFBP1 Regulate Tumor Immune and Drive Tumor Progression in Clear Cell Renal Cell Carcinoma.; Authors - Tianbo Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sareena (Verified Customer) (06-06-2023)

Works well when conjugated to fluorophores and tested with compensation beads

• Applications: Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: CD14 Monoclonal antibody

FH

Sunil (Verified Customer) (05-15-2023)

Great and fast delivery which is always useful!

• Applications: Flow Cytometry

FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Tris-EDTA antigen retrieval pH 9.0.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

CD14 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:60253-1-Ig)

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