Anticorps Recombinant de lapin anti-CD14

CD14 Recombinant Antibody for IF/ICC, FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IF/ICC, FC

Conjugaison

Non conjugué

CloneNo.

240225C2

N° de cat : 98020-1-RR

Synonymes

CD14 antigen, 240225C2

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Galerie de données de validation

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  IF Staining of RAW 264.7 using 98020-1-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (98020-1-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of mouse peritoneal macrophages using 98020-1-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR, Clone: 240225C2) and PE-conjugated Goat Anti-Rabbit IgG (red), or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98020-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98020-1-RR against Mouse CD14 were performed. The affinity constant is 0.383 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules RAW 264.7,
• Résultats positifs en cytométrie: macrophages péritonéaux de souris,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

98020-1-RR cible CD14 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD14 antigen
• Masse moléculaire calculée: 39 kDa
• Numéro d’acquisition GenBank: NM_009841.4
• Symbole du gène: Cd14
• Identification du gène (NCBI): 12475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

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