Anticorps Monoclonal anti-CD146/MCAM

CD146/MCAM Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

canin, Humain, Lapin, souris

Applications

WB, IF/ICC, FC

Conjugaison

Non conjugué

Publications(5)

CloneNo.

P1H12

N° de cat : 65181-1-Ig

Synonymes

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18



Applications testées

Résultats positifs en IF/ICCcellules HUVEC,
Résultats positifs en cytométriecellules HeLa,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65181-1-Ig cible CD146/MCAM dans les applications de WB, IF/ICC, FC et montre une réactivité avec des échantillons canin, Humain, Lapin, souris

Réactivité canin, Humain, Lapin, souris
Réactivité citéeHumain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène cellules ombilicales humaines cultivées
Nom complet melanoma cell adhesion molecule
Masse moléculaire calculée 646 aa, 72 kDa
Numéro d’acquisition GenBankBC056418
Symbole du gène CD146
Identification du gène (NCBI) 4162
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purifié par chromatographie d'affinité par protéine A
Tampon de stockage PBS with 0.09% sodium azide
Conditions de stockageStore at 2-8°C. Stable for one year after shipment.

Informations générales

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protocole

Product Specific Protocols
IF protocol for CD146/MCAM antibody 65181-1-IgDownload protocol
FC protocol for CD146/MCAM antibody 65181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Front Cell Dev Biol

Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.

Authors - Xun Xi
humanFC

Stem Cell Res Ther

Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models

Authors - Han Li
humanIF

J Mol Histol

Effects of simulated microgravity on dental pulp stem cell stemness

Authors - Huailong Hou
humanWB

Front Pharmacol

CD146 promotes resistance of NSCLC brain metastases to pemetrexed via the NF-κB signaling pathway

Authors - Hao Qu
humanFC

Biochim Biophys Acta Mol Cell Res

Senolytic elimination of senescent cells improved periodontal ligament stem cell-based bone regeneration partially through inhibiting YAP

Authors - Linglu Jia
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